Results using both methods correspond very well. The same serum samples are analyzed using GC-MS and spectrophotometry and the ratios of omega-6 to omega-3 obtained from the analyses are compared. An attempt to apply GSAM to serum samples is validated using GC-MS. The most encouraging algorithms employed are: coupling ridge regression (RR) with K-matrix, partial least squares regression (PLS), and generalized standard addition method (GSAM). Initially, calculations were made on spectral data that originated from prepared synthetic mixtures whose molar concentrations were known. To resolve the spectral data, a number of chemometric techniques were investigated. The visible spectrum for a typical plasma sample turned out to be the linear sum of the weighted contributions from all seven analytes that – given the heterogeneity of blood samples – leads to a broad diversity in the spectral patterns. Without it, a double or triple bond is not reactive. The critical moiety in the functional group is the α-methylene (allylic) group that triggers the reaction. The simple quantitative colorimetric assay is rapid, rugged, inexpensive and specific to the -CH=CH-CH2- functional group in both cyclic and acyclic structures. Included are cholesterol and the esters of linoleic, conjugated linoleic (CLA), arachidonic, linolenic, eicosapentaenoic, (EPA), and docosohexaenoic acids, (DHA). This article describes the development of a new paradigm in lipid testing wherein seven major human plasma lipids are determined simultaneously without the need for analytical separations.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |